specific reaction meaning in English
特殊反应
专化性反应
Examples
- Western - blotting result demonstrated rhpf4 had specific reaction with rabbit anti - hpf4 antibody
Western blotting检测表达产物与兔抗人hpf4抗体发生特异的抗原抗体反应。 - Recently , there has been rapid development in the research and application of biosensor , which can detect analytes selectively using the specific reaction of bioactive materials such as the enzyme - substrate , enzyme - coenzyme , antigen - antibody , incretion - acceptor and so on
近年来,生物传感器的研究和应用发展迅猛,它利用生物活性物质的亲和性,如酶-底物、酶-辅基、抗原-抗体、激素-受体等的分子识别功能,可以有选择地检测待测物。 - After sds - page and densitometric scan analysis , the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % . western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody , we got hng fusion protein and m - insulin fusion protein with purity of above 80 %
今士考个二目卜乙成功构建了ptxbi一hng及ptxbi一m一insulin原核表达克隆，并获得了高效表达，经过纯化得到纯度人于80 %的融合蛋白，并对人胰岛素突变体融合蛋白进行了初步活性测定。 - Ptxb1 - hng and ptxb1 - m - insulin are expressed in e . coli successfully . after sds - page and densitometric scan analysis , the results show that the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % of total bacterial proteins . western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody ( igg )
Pbv220 ? hng在大肠杆菌中未检测到表达，后两个克隆在大肠杆菌bl21 ( de3 )中获得高效表达， hng及m - insulin融合蛋白表达量分别占全菌蛋白的40及50左右；经western - blot鉴定m - insulin融合蛋白可以与小鼠抗人胰岛素单克隆抗体( igg )发生抗原抗体结合反应。 - Western - blotting result demostrated rhpf4 had specific reaction with rabbit anti - hpf4 antibody . our system improve the expression level of r hpf4 by 80 fold compared with pt7 - 7 - r hpf4 . after purified and renatured , r hpf4 prepared by our methods has bioactivity like wide hpf4 . our study establish a stable base for further reseach of the h pf4 and provide a theoretics gist for modulative mechanism of eukaryotic protein expression in prokaryotic cells
我们构建的rhpn原核高效表达系统经m page及凝胶密度扫描分析结果表明， rhpf4表达量占菌体总蛋白量的25 30 ，较原表达克隆pt7 7 rhpf4提高了近80倍，经快速高效的包涵体分高纯化工艺和复性工艺， rhpf4具有野生蛋白活性。